Biology 1009 - Lab - Rebecca Teed
Enzymes - Study Sheet
Three factors that influence the rate of a reaction are -
heat (kinetic energy)
- the concentrations of reactant and
product
- the presence of a catalyst
A catalyst decreases the activation
energy needed for a reaction by increasing the proportion of
collisions between substrate molecules which result in reactions.
They tend only to affect certain reactions in a system. An enzyme is
a protein catalyst. Amylase is the enzyme that catalyzes the
breakdown of starch into glucose and maltose. Amylase activity is
affected by: - time
- substrate concentration
- enzyme concentration
- pH
- temperature
In the lab book, be able to fulfill the objectives on p. 53 and be
certain that you understand the graphs on p. 48-51. For enzyme
concepts, you may also review the text (pp. 97 - 103), although all
the interesting stuff that wasn't in your lab book (such as inhibition
mechanisms) won't be on the quiz. There is a nice picture of an
enzyme, showing the three-dimensional structure and an active
site, on p. 99.
Additionally:
- How did you use the spectrophotometer to determine the activity
of amylase?
- What was the standardization curve for?
- Be able to explain how enzyme works in terms of active sites, E-S
complexes, etc.
- Know what these words mean:
- catalyst
- protein
- hydrogen bonds
- active site
- pH
- to denature
Return to the Biology 1009 Page.